DNA

Part:BBa_G0007

Designed by: Reshma Shetty   Group: iGEM07_Example   (2007-07-23)


Short sequence to promoter addition of 3' A overhang during PCR

This is a short sequence to promoter addition of 3' A overhang during PCR. It should be added to the 5' end of any primers in which the PCR product is going to be TA cloned. It is also a helpful spacer sequence when generating linear DNA fragments to ensure that restriction enzymes will still be able to cut.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None