Reporter

Part:BBa_E0666

Designed by: drew   Group: Antiquity   (2004-08-13)


CRY-FP output plasmid

R0011 driving CFP protein generator. R0051 driving mRFP-1 protein generator. R0040 driving YFP protein generator.


Usage and Biology

Take variants of a jellyfish protein that have the best spread on excitation and emission spectra and try them out on a single plasmid. If this works it will serve as an exhaustive reporter system for things like Elowitz's repressilator (i.e., lac, tet, lambda operators control FP expression)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2527
    Illegal AgeI site found at 2639
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/reporter
Parameters
None