Part:BBa_E0241

PoPS to GFP converter

This part accepts input PoPS and outputs untagged GFP. The part is very similar to E0240 but uses a T7 RNAP transcription terminator rather than an E. coli RNAP transcription terminator. This part will be used in a reporter device for dedicated transcription systems.

Usage and Biology

The sequence is good but there is a C inserted in the PstI site. The C is inserted immediately after the first C in the PstI sequence so there is still a valid PstI site.

This part has been shown to function as part of E7104

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 747
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 663