Difference between revisions of "Template:TypeIIS/Plasmids"

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====pOdd Loop Vectors (for Level 1)====
 
====pOdd Loop Vectors (for Level 1)====
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When four Level 0 parts are assembled into a Transcriptional Unit (TU) into the following vectors, they will be flanked by SapI restriction sites and their 3 bp Fusion Sites.
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When four Level 0 parts are assembled into a Transcriptional Unit (TU) into the following plasmid backbones (with <partinfo>BBa_J04454</partinfo>), the TU will be flanked by SapI restriction sites and these 3 bp Fusion Sites.
  
 
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Revision as of 19:19, 15 June 2020

Universal Acceptor Vector (for Level 0)


pOdd Loop Vectors (for Level 1)

When four Level 0 parts are assembled into a Transcriptional Unit (TU) into the following plasmid backbones (with BBa_J04454), the TU will be flanked by SapI restriction sites and these 3 bp Fusion Sites.

Registry Name Loop Name Fusion Site 5' Transcriptional Unit (TU) Fusion Site 3'
pSB1K01 pOdd1 ATG TU 1 GCA
pSB1K02 pOdd2 GCA TU 2 TAC
pSB1K03 pOdd3 TAC TU 3 CAG
pSB1K04 pOdd4 CAG TU 4 GGT


pEven Loop Vectors (for Level 2)