Difference between revisions of "Protein domains/Overview"

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*alter the protein's degradation rate (degradation tags)
 
*alter the protein's degradation rate (degradation tags)
 
*target the protein for cleavage (cleavage sites)
 
*target the protein for cleavage (cleavage sites)
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 +
Protein tags may include a start codon or a stop codon, but not both, since they are intended to be assembled in frame with protein coding sequences.

Revision as of 02:55, 17 November 2008

Protein tags and modifiers are short peptide sequences cloned in frame with protein coding sequences that change the protein's behavior.

Protein tags and modifiers might

  • change the protein's location (localization signals)
  • enable it to be readily purified (affinity tags)
  • alter the protein's degradation rate (degradation tags)
  • target the protein for cleavage (cleavage sites)

Protein tags may include a start codon or a stop codon, but not both, since they are intended to be assembled in frame with protein coding sequences.