Difference between revisions of "Part:pSB1AC3"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | Chloramphenicol resistant up to 3500 ug/ml. Tetracycline sensitive at 1.5 ug/ml. Kanamycin sensitive at 5 ug/ml (spayne and tk 6/6/05) | + | *Chloramphenicol resistant up to 3500 ug/ml. Tetracycline sensitive at 1.5 ug/ml. Kanamycin sensitive at 5 ug/ml (spayne and tk 6/6/05) |
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| + | *Primers for these Biobrick vectors can be found in [[Part:BBa_G00100]] (aka VF2) and [[Part:BBa_G00101]] (aka. VR) | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 21:49, 10 June 2007
High copy BioBrick assembly plasmid
pSB1AC3 is a high copy number plasmid carrying ampicillin and chloramphenicol resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). For the version of this plasmid with a CcdB insert, see BBa_P1010.
Annotated [http://www.biology.utah.edu/jorgensen/wayned/ape/ APE] file for pSB1AC3
Usage and Biology
- Chloramphenicol resistant up to 3500 ug/ml. Tetracycline sensitive at 1.5 ug/ml. Kanamycin sensitive at 5 ug/ml (spayne and tk 6/6/05)
- Primers for these Biobrick vectors can be found in Part:BBa_G00100 (aka VF2) and Part:BBa_G00101 (aka. VR)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3034
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3040 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3034
Illegal XhoI site found at 1035
Illegal XhoI site found at 1927 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 3034
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3034
Plasmid lacks a suffix.
Illegal XbaI site found at 3049
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2073