Part:BBa_Y00029

S. pombe homolog of S.cerevisiae SGF29, recoded for expression in S.c.

Y00029 is S. pombe gene SPBC1921.07c [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=protein&cmd=search&term=NP_596000.2] recoded for expression in S. cerevisiae. The pombe gene is the homolog of the cerevisiae SGF29 [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=sgf29], a subunit of the ATP-independent chromatin remodelling complex SAGA. Deletion of this gene in S. cerevisiae results in a viable strain. Requirements for SGF29 in SAGA function are largely unexplored. Unlike many of the SAGA subunits, the pombe and the cerevisiae gene encode proteins of abour the same size (259 aa in cerevisiae and 244 in pombe). The gene has been recoded for expression in S. cerevisiae by DNA 2.0, although it's not clear that this recoding will be necessary for expresssion. The recoded gene is 78% identical to the original pombe gene (Identities = 568/723).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 9
  Illegal suffix found in sequence at 766
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 9
  Illegal SpeI site found at 767
  Illegal PstI site found at 781
  Illegal NotI site found at 15
  Illegal NotI site found at 774
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 9
  Illegal BglII site found at 527
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 9
  Illegal suffix found in sequence at 767
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 9
  Illegal XbaI site found at 24
  Illegal SpeI site found at 767
  Illegal PstI site found at 781
  
• 1000
  COMPATIBLE WITH RFC[1000]