Protein_Domain

Part:BBa_T2025

Designed by: Reshma Shetty   Group: iGEM07_Example   (2009-09-17)

Glutathione S-transferase internal domain

This is a GST internal domain designed to be between protein domains. GST domains are a type of affinity domain that can be used to purify and detect of proteins.

Usage and Biology

The glutathione S-transferase (GST) protein domain enables one-step non-denaturing purification of proteins using a glutathione-agarose column. The GST protein domain can be places on either the N- or C-terminus of proteins. In some cases, fusion to GST can increase the expression and/or solubility of the fused protein. GST can also be used to immobilize proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/affinity
//proteindomain/internal/special
Parameters
None