Difference between revisions of "Part:BBa T2016:Design"
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===References=== | ===References=== | ||
Latest revision as of 13:12, 3 September 2009
S-tag head domain (KETAAAKFERQHMDS)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 16
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 10
Design Notes
This head domain begins with Met-Lys to promote efficient translation of the protein in E. coli. Rare codons from various model organisms were avoided where possible.
Source
This is a synthetic sequence.
References
<biblio>
- Kim93 pmid=8453373
// appears to be original reference
- Kim94 pmid=8059949
- Fritze pmid=11044970
</biblio>