Difference between revisions of "Part:BBa T2015"

 
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<partinfo>BBa_T2015 short</partinfo>
 
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This is a S-tag tail domain designed to be at the C-terminus of a protein. S-tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.  
 
This is a S-tag tail domain designed to be at the C-terminus of a protein. S-tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.  
  
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===Usage and Biology===
 
===Usage and Biology===
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The S-tag binds to the S-fragment of RNase A and is eluted at low pH.
  
 
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Revision as of 20:44, 2 September 2009

S-tag tail domain (KETAAAKFERQHMDS)

This is a S-tag tail domain designed to be at the C-terminus of a protein. S-tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.

Usage and Biology

The S-tag binds to the S-fragment of RNase A and is eluted at low pH.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 10
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4