Difference between revisions of "Part:BBa T2014:Design"

(→‎Design Notes)
(→‎References)
Line 14: Line 14:
 
===References===
 
===References===
 
<biblio>
 
<biblio>
 +
#Schmidt93 pmid=8433964
 +
// this might be the original reference
 
#Schmidt pmid=8433964
 
#Schmidt pmid=8433964
 
#Skerra07 pmid=17571060
 
#Skerra07 pmid=17571060

Revision as of 19:44, 13 August 2009

Strep-tag II special internal domain (WSHPQFEK)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This domain encodes the Strep-tag sequence WRHPQFGG. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like E. coli, humans, mouse, B. subtilis, C. elegans, Streptomyces coelicolor and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.

Source

This is a synthetic sequence.

References

<biblio>

  1. Schmidt93 pmid=8433964

// this might be the original reference

  1. Schmidt pmid=8433964
  2. Skerra07 pmid=17571060
  3. Skerra00 pmid=11036648
  4. Skerra99 pmid=10796988
  5. Skerra96 pmid=8636976

</biblio>