Difference between revisions of "Part:BBa T2014:Design"

 
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__NOTOC__
 
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<partinfo>BBa_T2014 short</partinfo>
 
<partinfo>BBa_T2014 short</partinfo>
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===References===
 
===References===
 +
<biblio>
 +
#Skerra07 pmid=17571060
 +
#Skerra00 pmid=11036648
 +
#Skerra99 pmid=10796988
 +
#Skerra96 pmid=8636976
 +
</biblio>

Revision as of 18:53, 13 August 2009

Strep-tag II special internal domain (WSHPQFEK)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This domain encodes the Strep tag sequence WRHPQFGG. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like E. coli, humans, mouse, B. subtilis, C. elegans, Streptomyces coelicolor and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.


Source

This is a synthetic sequence.

References

<biblio>

  1. Skerra07 pmid=17571060
  2. Skerra00 pmid=11036648
  3. Skerra99 pmid=10796988
  4. Skerra96 pmid=8636976

</biblio>