Difference between revisions of "Part:BBa T2014"

Latest revision as of 20:21, 13 August 2009

Strep-tag II special internal domain (WSHPQFEK)

This is a Strep-tag special internal domain designed to be internal to a protein. Strep-tag II is typically used for detection, purification and immobilization of proteins.

Usage and Biology

Strep-tag II specifically binds to either core streptavidin or streptavidin variant StrepTactin. As the Strep-tag II binds reversibly to the same pocket of streptavidin where the natural ligand D-biotin is complexed, it can be used to purify corresponding fusion proteins on affinity columns with immobilized streptavidin.

The Strep-tag II has been engineered for use as either an N- or C-terminal tag; however; it has slightly reduced binding affinity relative to the Strep-tag to streptavidin. This shortcoming can be addressed at least in part by using the Strep-Tactin protein, a variant of Streptavidin, which has been engineered to have higher affinity for both the Strep-tag and Strep-tag II peptides. [See references for details.]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 ===Usage and Biology===
+''Strep''-tag II specifically binds to either core streptavidin or streptavidin variant StrepTactin. As the ''Strep''-tag II binds reversibly to the same pocket of streptavidin where the natural ligand D-biotin is complexed, it can be used to purify corresponding fusion proteins on affinity columns with immobilized streptavidin.
+The ''Strep''-tag II has been engineered for use as either an N- or C-terminal tag; however; it has slightly reduced binding affinity relative to the ''Strep''-tag to streptavidin.  This shortcoming can be addressed at least in part by using the Strep-Tactin protein, a variant of Streptavidin, which has been engineered to have higher affinity for both the ''Strep''-tag and ''Strep''-tag II peptides.  [See references for details.]
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