Difference between revisions of "Part:BBa T2014"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_T2014 short</partinfo> | <partinfo>BBa_T2014 short</partinfo> | ||
| − | This is a Strep tag tail domain designed to be internal to a protein. Strep tags are typically used for detection, purification and immobilization of proteins. | + | This is a Strep-tag tail domain designed to be internal to a protein. Strep-tags are typically used for detection, purification and immobilization of proteins. |
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| + | Strep-tag specifically binds to core streptavidin. As the Strep-tag binds reversibly to the same pocket of streptavidin where the natural ligand D-biotin is complexed, it can be used to purify corresponding fusion proteins on affinity | ||
| + | columns with immobilized streptavidin. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 19:20, 13 August 2009
Strep-tag II special internal domain (WSHPQFEK)
This is a Strep-tag tail domain designed to be internal to a protein. Strep-tags are typically used for detection, purification and immobilization of proteins.
Strep-tag specifically binds to core streptavidin. As the Strep-tag binds reversibly to the same pocket of streptavidin where the natural ligand D-biotin is complexed, it can be used to purify corresponding fusion proteins on affinity columns with immobilized streptavidin.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]