Difference between revisions of "Part:BBa T2012:Design"

(→‎References)
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#Voss pmid=9415448
 
#Voss pmid=9415448
 
#Skerra07 pmid=17571060
 
#Skerra07 pmid=17571060
−
// includes brief overview of Strep-tag system
+
// includes brief overview of ''Strep''-tag system
 
#Skerra00 pmid=11036648
 
#Skerra00 pmid=11036648
 +
// history of ''Strep''-tag system
 
#Skerra99 pmid=10796988
 
#Skerra99 pmid=10796988
 
#Skerra96 pmid=8636976
 
#Skerra96 pmid=8636976
 
</biblio>
 
</biblio>

Revision as of 19:55, 13 August 2009

Strep-tag tail domain (WRHPQFGG)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This domain encodes the Strep-tag sequence WRHPQFGG. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like E. coli, humans, mouse, B. subtilis, C. elegans, Streptomyces coelicolor and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.

Source

This is a synthetic sequence.

References

<biblio>

  1. Schmidt93 pmid=8433964

// this looks to be the original reference for the Strep-tag

  1. Schmidt pmid=8433964
  2. Voss pmid=9415448
  3. Skerra07 pmid=17571060

// includes brief overview of Strep-tag system

  1. Skerra00 pmid=11036648

// history of Strep-tag system

  1. Skerra99 pmid=10796988
  2. Skerra96 pmid=8636976

</biblio>