Protein_Domain
c-Myc

Part:BBa_T2008

Designed by: Reshma Shetty   Group: iGEM07_Example   (2009-08-11)

c-Myc special internal domain (EQKLISEEDL)

This is a c-Myc tag special internal domain designed to be within a protein. c-Myc tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.

Usage and Biology

The c-myc tag can be used at the N- or C-terminus or even internal to proteins. The c-myc-tag has been used in western blots, immunoprecipitation, flow cytometry, and affinity purification. Proteins with the c-myc-tag can be affinity-purified by coupling the murin anti-c-myc antibody 9E10 to divinyl sulphone -activated agarose. Note that the low pH elution conditions used during affinity purification may impact protein properties and that the matrix can not be reused efficiently. Thus, c-myc-tags are more commonly used for protein detection rather than purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/affinity
//proteindomain/internal/special
Parameters
None