Difference between revisions of "Part:BBa T2005"

Latest revision as of 21:23, 14 August 2009

FLAG octapeptide special internal domain (DYKDDDDK)

This is a FLAG tag octapeptide special internal domain designed to be internal to the sequence of a protein. FLAG tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.

Usage and Biology

The FLAG tag can be used at either the N- or C-terminus of a protein and can be used to purify protein from bacteria, yeast and mammalian cells using monoclonal antibody bound to a solid support. FLAG tags support nondenaturing purification of proteins. Note, however, that the monoclonal antibody matrix used for purification is not as stable as a Ni-NTA or StrepTactin column and cannot be regenerated for reuse.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_T2005 short</partinfo>
@@ Line 5: / Line 4: @@
 This is a FLAG tag octapeptide special internal domain designed to be internal to the sequence of a protein. FLAG tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+The FLAG tag can be used at either the N- or C-terminus of a protein and can be used to purify protein from bacteria, yeast and mammalian cells using monoclonal antibody bound to a solid support.  FLAG tags support nondenaturing purification of proteins.  Note, however, that the monoclonal antibody matrix used for purification is not as stable as a Ni-NTA or StrepTactin column and cannot be regenerated for reuse.
 <!-- -->