Protein_Domain
FLAG

Part:BBa_T2005

Designed by: Reshma Shetty   Group: iGEM07_Example   (2009-08-11)

FLAG octapeptide special internal domain (DYKDDDDK)

This is a FLAG tag octapeptide special internal domain designed to be internal to the sequence of a protein. FLAG tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.

Usage and Biology

The FLAG tag can be used at either the N- or C-terminus of a protein and can be used to purify protein from bacteria, yeast and mammalian cells using monoclonal antibody bound to a solid support. FLAG tags support nondenaturing purification of proteins. Note, however, that the monoclonal antibody matrix used for purification is not as stable as a Ni-NTA or StrepTactin column and cannot be regenerated for reuse.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/affinity
//proteindomain/internal/special
Parameters
None