Difference between revisions of "Part:BBa T2004:Design"

(→‎Design Notes)
 
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===References===
 
===References===
 
<biblio>
 
<biblio>
 +
#Hopp Thomas P. Hopp, Kathryn S. Prickett, Virginia L. Price, Randell T. Libby, Carl J. March1, Douglas Pat Cerretti1, David L. Urdal1 & Paul J. Conlon1.  ''A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification.''  Bio/Technology 1988 Oct; 6():1204-1210. doi:10.1038/nbt1088-1204
 +
// original reference
 +
#Terpe pmid=12536251
 
#Chubet pmid=8770418
 
#Chubet pmid=8770418
 
</biblio>
 
</biblio>
 
*[http://www-users.med.cornell.edu/~jawagne/FLAG-tag.html Addition of an epitope to a protein coding sequence: FLAG-tag]
 
*[http://www-users.med.cornell.edu/~jawagne/FLAG-tag.html Addition of an epitope to a protein coding sequence: FLAG-tag]

Latest revision as of 21:32, 14 August 2009

FLAG octapeptide head domain (DYKDDDDK)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The FLAG sequence AspTyrLysAspAspAspAspLys (DYKDDDDK) is one of the most commonly used FLAG tag sequences. Each of the amino acids in this octapeptide have two possible codon choices. For each amino acid, neither of the two codon choices appear to be rare in the common model organisms. So codons were selected to minimize repeats.

An ATG start codon was used followed by a AAA second codon. AAA is the preferred codon for the second codon position in E. coli.

Source

This is a synthetic sequence.

References

<biblio>

  1. Hopp Thomas P. Hopp, Kathryn S. Prickett, Virginia L. Price, Randell T. Libby, Carl J. March1, Douglas Pat Cerretti1, David L. Urdal1 & Paul J. Conlon1. A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification. Bio/Technology 1988 Oct; 6():1204-1210. doi:10.1038/nbt1088-1204

// original reference

  1. Terpe pmid=12536251
  2. Chubet pmid=8770418

</biblio>

  • [http://www-users.med.cornell.edu/~jawagne/FLAG-tag.html Addition of an epitope to a protein coding sequence: FLAG-tag]