Part:BBa_T2004
FLAG octapeptide head domain (DYKDDDDK)
This is a FLAG tag octapeptide head domain designed to be at the N-terminus of a protein. FLAG tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
Usage and Biology
The FLAG tag can be used at either the N- or C-terminus of a protein and can be used to purify protein from bacteria, yeast and mammalian cells using monoclonal antibody bound to a solid support. FLAG tags support nondenaturing purification of proteins. Conveniently, FLAG head domains can be cleaved off immediately after the C-terminal Lysine using enterokinase. Note, however, that the monoclonal antibody matrix used for purification is not as stable as a Ni-NTA or StrepTactin column and cannot be regenerated for reuse.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
//proteindomain/head
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