Difference between revisions of "Part:BBa T2002:Design"
 
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===References===
 
===References===
 +
 +
<biblio>
 +
#Bornhorst pmid=11036646
 +
</biblio>

Latest revision as of 20:43, 14 August 2009

7x His tag special internal domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a 7x His tag rather than a 6x His tag because Tom Knight had heard anecdotally that 7x His is more effective than 6x His.

Used both Histidine codons to minimize repeats.

Source

This is a synthetic sequence.

References

<biblio>

  1. Bornhorst pmid=11036646

</biblio>