Difference between revisions of "Part:BBa T2000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This is a 7x His tag rather than a 6x His tag because Tom Knight had heard anecdotally that 7x His is more effective than 6x His. | + | This is a 7x His tag rather than a standard 6x His tag because Tom Knight had heard anecdotally that 7x His is more effective than 6x His. |
Used both His codons in order to avoid repeats. Also included a double TAATAA stop codon. | Used both His codons in order to avoid repeats. Also included a double TAATAA stop codon. | ||
Revision as of 15:24, 13 August 2009
7x His tag tail domain
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is a 7x His tag rather than a standard 6x His tag because Tom Knight had heard anecdotally that 7x His is more effective than 6x His.
Used both His codons in order to avoid repeats. Also included a double TAATAA stop codon.
Source
This is a synthetic sequence.