Regulatory
Zif23

Part:BBa_R2000

Designed by: Ronny Krashinsky   Group: Antiquity   (2004-01-29)


Promoter, Zif23 regulated, test: between

Promoter with standard -10 and -35 sites. A symmetric Zif23 dimer target site is between the -10 and -35 sites.


Usage and Biology

See [Wolfe00] regarding Zif23.

This part successfully promotes transcription of GFP when placed upstream of BBa_E0240 or BBa_E0840. It is a very strong promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/negative
//classic/regulatory/uncategorized
Parameters
biology
directionForward
negative_regulators1
positive_regulators