Regulatory
cII p22

Part:BBa_R1053

Designed by: Maia Mahoney, Drew Endy   Group: Antiquity   (2003-01-31)


Promoter, Standard (p22 cII regulated)

[Note: This is the same part as R0053 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001].

The p22 cII regulatory region sequence is a 97 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. p22 cII repressor protein, BBa_C0053, binds to it.

This segment contains O-R1, O-R2, a fragment of O-R3, the -35 of P-RM, and P-R (-10 and -35 from Tom Knight)


Usage and Biology

p22 cII is a member of the lamboid cI protein family. Weak-medium promoter. May not be well matched with other '10 series' promoters. [jb, 5/24/04]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/negative
//classic/regulatory/uncategorized
Parameters
negative_regulators1
positive_regulators