Generator
ccdB

Part:BBa_P1010

Designed by: Leon Chan   Group: Antiquity   (2004-07-28)
Revision as of 20:04, 9 June 2006 by Smelissali (Talk | contribs) (BioBrick Plasmids Containing This Part)

ccdB cell death gene

CcdB Plasmid.png

The CcdB protein, constitutively expressed by P1010, is lethal to most of the BioBrick cell strains, only DB3.1 is resistant.

P1010 is used when putting BioBrick parts into BioBrick plasmids. The part to be inserted and the plasmid are cut with BioBrick enzymes and mixed. The mixture will include both the original uncut or religated plasmid and the desired structure. However, because of CcdB, all of the cells containing the original plasmid die and the surviving colonies are the desired result.

BioBrick Construction Plasmids Containing This Part

Most BioBrick plasmids are delivered with the P1010 insert see P1010 Physical DNA for the current list of plasmids that are available in this form. This particular Biobrick with its Plasmid backbone is considered a Construction Plasmid.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 664
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 114
[edit]
Categories
//function/celldeath
//plasmidbackbone/component/defaultinsert
Parameters
o_h
proteinCcdB
rbs
tagNone