Generator
ccdB

Part:BBa_P1010

Designed by: Leon Chan   Group: Antiquity   (2004-07-28)
Revision as of 20:44, 8 June 2006 by Randy (Talk | contribs)

ccdB cell death gene

CcdB Plasmid.png

The CcdB protein, constitutively expressed by P1010, is lethal to most of the BioBrick cell strains, only DB3.1 is resistant.

P1010 is used when putting BioBrick parts into BioBrick plasmids. Most BioBrick plasmids are delivered with the P1010 insert see Physical DNA for the current list of plasmids that are available in this form.

BioBrick Plasmids Containing This Part

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 664
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 114
[edit]
Categories
//function/celldeath
//plasmidbackbone/component/defaultinsert
Parameters
o_h
proteinCcdB
rbs
tagNone