RBS
BD14

Part:BBa_M36204

Designed by: Steven Lee   Group: Stanford BIOE44 - S11   (2011-12-06)


Bicistronic RBS (BD14)

This is a bicistronic RBS, consisting of a constitutive RBS, followed by a leader sequence that includes the second RBS.

The first constitutive RBS uses ribosomes to physically occupy the second RBS, preventing the second RBS from binding to genes of interest downstream. This ensures a more consistent, stable expression level of your gene of interest.

This bicistronic RBS was designed by Andrew Endy. More information can be found at BIOFAB.org.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None