Device
tuned pArs

Part:BBa_K935002:Experience

Designed by: Twaddle Lab   Group: iGEM12_IvyTech-South_Bend   (2012-10-02)
Revision as of 14:54, 6 October 2012 by Geotwaddle (Talk | contribs) (→‎Applications of BBa_K935002)

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Response of BBa_935002 in E.coli.

A dilution series of sodium arsenate was created in 100 uL of LB broth in 96-well tissue culture plates. Replicate 100ul of cells were plated at a density of 2.5*10^8 cells per mL and incubated for 12 hrs at 37C. Epifluoresence (Ex518/Em607) was measured on a Molecular Devices FilterMax F3 reader (PMT 2 sec) and divided by the cell number determined by an A600 scan of the same wells to derive the flourescence per cell (which was multiplied by 10^4 for charting convenience). Error bars represent standard deviation of the sample mean.

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