Coding

Part:BBa_K899001

Designed by: Andreas Kjr   Group: iGEM12_SDU-Denmark   (2012-09-22)

Fructan fructan fructosyltransferase (FFT)

This part is the coding sequence for Fructan:Fructan 1-Fructosyltransferase isolated from jerusalem artichoke mRNA. This enzyme elongates a trisaccharide with the fructose unit of a sucrose substrate into an inulin polymer via the reaction [G-Fm + GFn ↔ G-F(m-1) + G-F(n+1) with m > 1 and n ≥ 1] Works together with part BBa_K899000 (SST). To view the characterization of this part please refer to [1] which is the same as this part but with a "double" RBS containing both the shine-dalgarno and kozak sequence [2].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 515
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 369
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/probiotics/production
Parameters
None