Coding

Part:BBa_K899000

Designed by: Andreas Kjr   Group: iGEM12_SDU-Denmark   (2012-09-22)

Sucrose sucrose fructosyltransferase (SST)

This part is the coding sequence for Sucrose:sucrose 1-fructosyltransferase isolated from jerusalem artichoke mRNA.

It Catalyzes sucrose to the three products: D-sucrose, beta-D-fructofuranosyl-(2->1) and alpha-D-glucopyranoside, as such:

2 sucrose -> D-glucose + beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl + alpha-D-glucopyranoside.

To view the characterization of this part please refer to [1] which is the same as this part but with a "double" RBS containing both the shine-dalgarno and kozak sequence [2].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1826
    Illegal BamHI site found at 119
    Illegal BamHI site found at 609
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 70
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/probiotics/production
Parameters
None