Part:BBa_K879024
rbs 4
The Salis RBS calculator was used to design this ribosome binding site (https://salis.psu.edu/software/). It is one of several RBS sequences of similar strength that were each tailored to a particular ORF, in this case, the lambda Cl repressor (BBa_K879016), and that showed significant sequence variation between each other to minimize the chances of intra-molecular recombination when used in combination. The RBS was calculated for each protein coding sequence with a target translation initiation strength set to a value of 50,000 for each of the ORFs used by the 2012 University of Alberta iGEM team using a common length constraint that began with the common transcriptional start site. The level of expression observed using this RBS for the lambda Cl repressor was not observed.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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