Difference between revisions of "Part:BBa K863206"

Revision as of 03:37, 27 October 2012

Mating factor alpha 1 with Kozak sequence

With a N-terminal mating factor alpha 1 from Saccharomyces sereviceae linked to the protein of interesst, it could secreted into the media. This allows a better purification of the protein.

This part is a part of a shuttle vector (K863204) that could use for site directed recombination in the yeast P. pastoris.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 256
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K863206 short</partinfo>
-With an N-terminal mating factor alpha 1 from ''Saccharomyces sereviceae'' linked to the protein of interesst, it could secreted into the media. This allows a better purification of the protein.
+With a N-terminal mating factor alpha 1 from ''Saccharomyces sereviceae'' linked to the protein of interesst, it could secreted into the media. This allows a better purification of the protein.
+This part is a part of a shuttle vector ([https://parts.igem.org/Part:BBa_K863204 K863204]) that could use for site directed recombination in the yeast ''P. pastoris''.
 <!-- Add more about the biology of this part here