Difference between revisions of "Part:BBa K863205"
 
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<partinfo>BBa_K863205 short</partinfo>
 
<partinfo>BBa_K863205 short</partinfo>
  
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GFP was amplified by PCR and cloned via the ''Aar''I restriction site in the shuttle vector pECPP11JS [https://parts.igem.org/Part:BBa_K863204 K863204].
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:42, 27 September 2012

GFP integrated in shuttle vector pECPP11JS (BBa_K863204) for integration in yeast

GFP was amplified by PCR and cloned via the AarI restriction site in the shuttle vector pECPP11JS K863204.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4772
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4067
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3
    Illegal BglII site found at 2218
    Illegal BamHI site found at 2575
    Illegal XhoI site found at 1198
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4772
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4772
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4928
    Illegal BsaI.rc site found at 1862
    Illegal BsaI.rc site found at 2727