Difference between revisions of "Part:BBa K863202"

 
 
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<partinfo>BBa_K863202 short</partinfo>
 
<partinfo>BBa_K863202 short</partinfo>
  
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his4 gene from Pichia pastoris
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The gene ''his4'' from ''Pichia pastoris'' encode a trifunctional enzyme catalyzing the second (phosphoribosyl-ATP pyrophosphohydrolase), third (phosphoribosyl-AMP cyclohydrolase) and tenth (histidinol dehydrogenase) steps in histidine biosynthesis.
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This part is a part of a shuttle vector ([https://parts.igem.org/Part:BBa_K863204 K863204]) that could use for site directed recombination in the yeast ''P. pastoris''.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:43, 27 October 2012

his4 gene from Pichia pastoris

The gene his4 from Pichia pastoris encode a trifunctional enzyme catalyzing the second (phosphoribosyl-ATP pyrophosphohydrolase), third (phosphoribosyl-AMP cyclohydrolase) and tenth (histidinol dehydrogenase) steps in histidine biosynthesis.

This part is a part of a shuttle vector (K863204) that could use for site directed recombination in the yeast P. pastoris.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2555
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1850
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BamHI site found at 358
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2555
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2555
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 510