DNA

Part:BBa_K863201:Design

Designed by: Julia Schirmacher   Group: iGEM12_Bielefeld-Germany   (2012-09-23)
Revision as of 02:22, 27 September 2012 by JSchirmacher (Talk | contribs) (→‎Source)

3' UTR site of alcohol oxidase 1 gene (aox1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 59


Design Notes

Primers for isolation of the sequence with BioBrick Prefix in the fwd primer and Suffix in the rev primer.
fwd: 5'-ACGTgaattcgcggccgcttctagagCGAGTATCTATGATTGGAA-3'
rev: 5'-ACGTCTGCAGCGGCCGCTACTAGTAAAAACAAGATAGTGCCCCTC-3'

Source

References