Other
Part:BBa_K863200:Design
Designed by: Julia Schirmacher Group: iGEM12_Bielefeld-Germany (2012-09-20)
5' UTR site of alcohol oxidase 1 gene (aox1)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers for isolation of the sequence with BioBrick Prefix in the fwd primer and Suffix in the rev primer.
fwd: 5'-ACGTgaattcgcggccgcttctagagAGATCTAACATCCAAAGACG-3'
rev: 5'-ACGTCTGCAGCGGCCGCTACTAGTACGTTTCGAATAATTAGTTGT-3'
Source
Pichia pastoris wild type X-33