Difference between revisions of "Part:BBa K863122"

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GFP with constant promoter and His-tag made from [https://parts.igem.org/Part:BBa_I13522 BBa_I13522] (without the terminators).
 
GFP with constant promoter and His-tag made from [https://parts.igem.org/Part:BBa_I13522 BBa_I13522] (without the terminators).
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Originally designed to have a comparison to a His-tagged GFP to cellulose binding domain fusion protein.
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===Usage and Biology===
 
===Usage and Biology===
  

Latest revision as of 22:03, 26 September 2012

BBa_I13522 with His-tag

GFP with constant promoter and His-tag made from BBa_I13522 (without the terminators). Originally designed to have a comparison to a His-tagged GFP to cellulose binding domain fusion protein.

Usage and Biology

GFP (mut3b) [note that this part does not have a barcode]

Tagged version of gfp from Repressilator reporter. See the design page for more source information.


Fluorescence wavelengths

Cormack et al.Cormack report the following excitation and emission data for GFPmut3 -

  • Excitation max - 501nm
  • Emission max - 511nm

Latency

Cormack et al.Cormack report detectable fluorescence within 8 mins.

Pictures

  • BBa_I13522 visualized under non-UV lightbox

  • BBa_I13522 visualized under 254nm wavelength UV lightbox

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 813
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724