Coding
GFP_His
Part:BBa_K863121:Design
Designed by: Moritz Müller Group: iGEM12_Bielefeld-Germany (2012-09-20)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (His-tag)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 742 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 653
Design Notes
This BioBrick is meant to be a reporter-domain. It can be fused with the Standard 25 assembly (Freiburg). At the C-terminus a His6-tag was added for a easy capturing of the fusion protein.
Primers that were used to make this BioBrick:
GFP_Frei | TACGGAATTCGCGGCCGCTTCTAGATGGCCGGCATGCGTAAAGGAGAAGAACTT |
GFP_His6_compl | CTGCAGCGGCCGCTACTAGTATTAACCGGTGTGATGGTGATGGTGATGTTTGTATAGTTCATCCATGCCATGTG |