Coding
GFP_His

Part:BBa_K863121:Design

Designed by: Moritz Müller   Group: iGEM12_Bielefeld-Germany   (2012-09-20)
Revision as of 22:13, 26 September 2012 by Mo (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (His-tag)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 742
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 653


Design Notes

This BioBrick is meant to be a reporter-domain. It can be fused with the Standard 25 assembly (Freiburg). At the C-terminus a His6-tag was added for a easy capturing of the fusion protein.

Primers that were used to make this BioBrick:

GFP_Frei TACGGAATTCGCGGCCGCTTCTAGATGGCCGGCATGCGTAAAGGAGAAGAACTT
GFP_His6_compl CTGCAGCGGCCGCTACTAGTATTAACCGGTGTGATGGTGATGGTGATGTTTGTATAGTTCATCCATGCCATGTG

Source

BBa_I13522

References