Difference between revisions of "Part:BBa K863121"
 
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<partinfo>BBa_K863121 short</partinfo>
 
<partinfo>BBa_K863121 short</partinfo>
  
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GFP from Repressilator reporter. See the [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0040 untagged version] for source information.  
+
GFP from Repressilator reporter in Standard 25 (Freiburg) See the [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0040 untagged version] for source information.  
  
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 22:08, 26 September 2012

green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (His-tag)

GFP from Repressilator reporter in Standard 25 (Freiburg) See the untagged version for source information.

Usage and Biology

GFP (mut3b) [note that this part does not have a barcode]

Tagged version of gfp from Repressilator reporter. See the design page for more source information.


Fluorescence wavelengths

Cormack et al.Cormack report the following excitation and emission data for GFPmut3 -

  • Excitation max - 501nm
  • Emission max - 511nm

Latency

Cormack et al.Cormack report detectable fluorescence within 8 mins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 742
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 653