Difference between revisions of "Part:BBa K863120"

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<partinfo>BBa_K863120 short</partinfo>
 
<partinfo>BBa_K863120 short</partinfo>
  
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GFP from Repressilator reporter in Standard 25 (Freiburg) See the [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0040 untagged version] for source information.  
+
GFP from Repressilator reporter in Standard 25 (Freiburg) See the [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0040 original version] for source information.  
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 22:47, 26 September 2012

green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (Freiburg-Standard)

GFP from Repressilator reporter in Standard 25 (Freiburg) See the original version for source information.

Usage and Biology

GFP (mut3b)

Untagged version of gfp from Repressilator reporter. See the design page for more source information.

BioBrick has not yet been used. Built to be fused to binding domains for characterization. Standard 25 restriction-sites are at both ends to switch order of GFP and fused protein.


Fluorescence wavelengths

Cormack et al.Cormack report the following excitation and emission data for GFPmut3 -

  • Excitation max - 501nm
  • Emission max - 511nm

Latency

Cormack et al.Cormack report detectable fluorescence within 8 mins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 721
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 650