Difference between revisions of "Part:BBa K863120"
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<partinfo>BBa_K863120 short</partinfo>
 
<partinfo>BBa_K863120 short</partinfo>
  
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GFP from Repressilator reporter. See the [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0040 original version] for source information.  
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GFP from Repressilator reporter in Standard 25 (Freiburg) See the [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0040 untagged version] for source information.  
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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GFP (mut3b) [note that this part does not have a barcode]
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Un[[Help:Tag|tag]]ged version of gfp from Repressilator reporter.  See the [[Part:BBa_E0040:Design | design page]] for more source information.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_E0040 SequenceAndFeatures</partinfo>
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<!-- -->
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<!--
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===Functional Parameters===
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<partinfo>BBa_E0040 parameters</partinfo>
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====Fluorescence wavelengths====
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Cormack ''et al''.<cite>Cormack</cite> report the following excitation and emission data for GFPmut3 - <br>
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*'''Excitation max''' - 501nm
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*'''Emission max''' - 511nm
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====Latency====
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Cormack ''et al''.<cite>Cormack</cite> report detectable fluorescence within 8 mins.
  
 
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Revision as of 22:41, 26 September 2012

green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (Freiburg-Standard)

GFP from Repressilator reporter in Standard 25 (Freiburg) See the untagged version for source information.

Usage and Biology

GFP (mut3b) [note that this part does not have a barcode]

Untagged version of gfp from Repressilator reporter. See the design page for more source information.


Fluorescence wavelengths

Cormack et al.Cormack report the following excitation and emission data for GFPmut3 -

  • Excitation max - 501nm
  • Emission max - 511nm

Latency

Cormack et al.Cormack report detectable fluorescence within 8 mins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 721
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 650