Reporter

Part:BBa_K863113:Design

Designed by: Moritz Müller   Group: iGEM12_Bielefeld-Germany   (2012-09-20)
Revision as of 20:49, 26 September 2012 by Mo (Talk | contribs) (→‎Source)

Cellulose binding Domain of C. cellulovorans cellulose binding protein with Reporter GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1067
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 978


Design Notes

CBDclos fused to a his-tagged GFP in a Freiburg-manner


Source

The origin of this part is a cloning-vector from the fermentation group of Bielefeld University (the cellulose domain) and BBa_K863121 as template for the GFP-fusion protein.

References