Difference between revisions of "Part:BBa K863104:Design"

(→‎Design Notes)
 
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This part is was made by a mixture of Freiburg-assembly of <partinfo>BBa_K863101</partinfo> and <partinfo>BBa_K863121</partinfo> and suffix insertion into <partinfo>BBa_J61101</partinfo>.
 
This part is was made by a mixture of Freiburg-assembly of <partinfo>BBa_K863101</partinfo> and <partinfo>BBa_K863121</partinfo> and suffix insertion into <partinfo>BBa_J61101</partinfo>.
 
Its purpose was to analyze of the expression problem of a similar construct with a T7-promoter.
 
Its purpose was to analyze of the expression problem of a similar construct with a T7-promoter.
 +
 +
The Biobrick was designed to express the cellulose binding domain of Cellulomonas fimi exoglucanase (CBDcex) with a fused GFP as a reporter protein with a constitutive promoter<partinfo>J23100</partinfo>. 12 additional conserved bases (4 AS) were isolated upstream of the cellulose binding domain and 9 bases downstream of the exoglucanase gene. A short C-terminal (Glycine, Serine) linker and the Freiburg-scar connect the CBD to GFP. For easy capturing a His-tag was added to the C-terminal end of the GFP.
  
 
===Source===
 
===Source===
−
The origin of the cellulose binding domain is a cloning vector of the fermantation group of Bielefeld University.
+
The origin of this part is a cloning-vector from the fermentation group of Bielefeld University (the cellulose domain) and [https://parts.igem.org/Part:BBa_K863121 BBa_K863121] as template for the GFP-fusion protein.  
  
 
===References===
 
===References===

Latest revision as of 20:31, 26 September 2012

Cellulose binding Domain from C. Fimi with const. Promotor and GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 37
    Illegal NgoMIV site found at 65
    Illegal AgeI site found at 1136
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1047


Design Notes

This part is was made by a mixture of Freiburg-assembly of BBa_K863101 and BBa_K863121 and suffix insertion into BBa_J61101. Its purpose was to analyze of the expression problem of a similar construct with a T7-promoter.

The Biobrick was designed to express the cellulose binding domain of Cellulomonas fimi exoglucanase (CBDcex) with a fused GFP as a reporter protein with a constitutive promoterBBa_J23100. 12 additional conserved bases (4 AS) were isolated upstream of the cellulose binding domain and 9 bases downstream of the exoglucanase gene. A short C-terminal (Glycine, Serine) linker and the Freiburg-scar connect the CBD to GFP. For easy capturing a His-tag was added to the C-terminal end of the GFP.

Source

The origin of this part is a cloning-vector from the fermentation group of Bielefeld University (the cellulose domain) and BBa_K863121 as template for the GFP-fusion protein.

References