Coding

Part:BBa_K863015

Designed by: Isabel Huber   Group: iGEM12_Bielefeld-Germany   (2012-09-20)


xccl laccase from Xanthomonas campestris with T7 promoter, RBS and His-tag

xccl laccase from Xanthomonas campestris with pT7 and HIS tag

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 196
    Illegal PstI site found at 1582
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 196
    Illegal PstI site found at 1582
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1131
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 196
    Illegal PstI site found at 1582
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 196
    Illegal PstI site found at 1582
    Illegal NgoMIV site found at 267
    Illegal NgoMIV site found at 372
    Illegal NgoMIV site found at 623
    Illegal NgoMIV site found at 816
    Illegal NgoMIV site found at 820
    Illegal NgoMIV site found at 1206
    Illegal NgoMIV site found at 1227
    Illegal AgeI site found at 554
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
//function/degradation
//proteindomain/degradation
Parameters
None