Composite

Part:BBa_K822004

Designed by: Jarle Pahr   Group: iGEM12_NTNU_Trondheim   (2012-09-25)
Revision as of 11:13, 25 September 2012 by Registry (Talk | contribs)

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LacI generator (no promoter)

A composite part with coding sequence for LacI-LVA together with ribosome binding site (RBS) and a double transcriptional terminator. Fore use in regulation of LacI-repressed genetic elements, by combining the part with an appropriate promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1168
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
Parameters
directionForward
functionRep
ligandsallolactose, IPTG
proteinLacI
swissproP03023
tagLVA