Difference between revisions of "Part:BBa K822004"

Revision as of 18:54, 26 September 2012

LacI generator (no promoter)

A composite part with coding sequence for LacI-LVA together with ribosome binding site (RBS) and a double transcriptional terminator. For use in regulation of LacI-repressed genetic elements, by combining the part with an appropriate promoter.

This part is a duplicate of BBa_K292006. The NTNU iGEM 2011 team found that the physical DNA available from the registry for that part did not match the registered sequence , and we have therefore remade it. Sequencing confirms that this part has the desired sequence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1168
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K822004 short</partinfo>
-A composite part with coding sequence for LacI-LVA together with ribosome binding site (RBS) and a double transcriptional terminator. Fore use in regulation of LacI-repressed genetic elements, by combining the part with an appropriate promoter.
+A composite part with coding sequence for LacI-LVA together with ribosome binding site (RBS) and a double transcriptional terminator. For use in regulation of LacI-repressed genetic elements, by combining the part with an appropriate promoter.
+This part is a duplicate of <partinfo>BBa_K292006</partinfo>. The NTNU iGEM 2011 team found that the physical DNA available from the registry for that part [https://parts.igem.org/Part:BBa_K292006:Experience did not match the registered sequence] , and we have therefore remade it. Sequencing confirms that this part has the desired sequence.