Part:BBa_K822003

Constitutive YFP generator

Yellow Fluorescent Protein coding sequence expressed under a constitutive promoter for use as positive control in fluorescence measurement experiments.

Testing:

The part was tested using a Tecan Infinite 200 Pro plate reader for fluorescence measurements. A culture of E. coli strain DH5a containing the part was incubated for ~24 h at 37 C with shaking. Cells containing a construct with GFP (BBa_K082003) under the Jen1 promoter (BBa_K284002) were not expected to express GFP under the conditions and were used as a negative control.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]