Coding
Part:BBa_K822002
Designed by: Jarle Pahr Group: iGEM12_NTNU_Trondheim (2012-09-23)
Colicin E1 + immunoprotein
Coding sequence for the channel-forming protein Colicin E1 of E. coli and its associated immunoprotein.
Testing:
The part was placed under a constitutive promoter and cell growth experiments performed to determine if cell growth would be inhibited in samples treated with lysate of the cells containing the part. Experiments showed a delayed response with differences between those samples treated with lysate from the putative colicin-producing cells and those treated with lysate from a negative control being apparent after about 3 hours.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 301
[edit]
Categories
Parameters
None |