Regulatory

Part:BBa_K822000

Designed by: Jarle Pahr   Group: iGEM12_NTNU_Trondheim   (2012-09-23)
Revision as of 14:45, 26 September 2012 by Jarlemag (Talk | contribs)

lldPRD operon promoter + RBS from E. coli

This is the promoter region for the lldPRD operon (previously named lct) of E. coli. The lldPRD operon encodes a lactate-dehydrogenase, and the lldPRD promoter has been shown to be induced by L-lactate.

The promoter is repressed/activated by the regulatory protein lldR encoded by the operon. In the absence of L-lactate, lldR binds to two operator sites O1 and O2 in the promoter region and inhibits expression. [1]

The activity of the promoter may be repressed by chloramphenicol and nutrient-rich growth environment. [2]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 335


References:

[1]: Dual role of lldR in regulation of the lldPRD operon, involved in L-lactate metabolism in E. coli. Aguilera et al. J. Bacteriol. 2008, 190(8):2997

[2]: Inducible Membrane-bound L-Lactate Dehydrogenase from Escherichia coli - purification and properties. Futai, M. and Kimura, H. Journal of Biological Chemistry. 1977, 252(16):5820-5827.

[edit]
Categories
Parameters
None