Coding

Part:BBa_K775003

Designed by: Sietske Grijseels   Group: iGEM12_TU-Delft   (2012-09-05)

RI7-odr10 (diacetyl) chimeric GPCR with promoter and terminator

BBa_K775003 encodes a chimeric diacetyl-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the C. elegans 7-transmembrane receptor ODR-10, flanked by the N- and C- terminals of the rat OR RI7. This part is an improvement of BBa_K211002 made by 2009 Hongkong university. A GPD promoter, Kozak sequence, and CYC1 terminator have been added.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 113
    Illegal XhoI site found at 885
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/yeast
Parameters
None